Fig. 6

Verification of PSAT1 expression levels. A PSAT1 mRNA levels are elevated in 20 pairs of ccRCC tissues compared with matched tissues. B PSAT1 protein levels in 20 pairs of ccRCC tissues and matched noncancerous tissues were detected by WB. 20 pairs ccRCC samples: (10 pairs: late-stage; 10 pairs: early-stage). The abscissa represents the sample type, and the ordinate represents the relative expression values of mRNA or protein levels in tumor tissues compared with adjacent normal tissues. The level of the columns represents the level of expression. qRT-PCR and WB data are presented as the mean ± SD. (N: noncancerous, T: tumor; ****P < 0.0001, ***P < 0.001; ns: no significance). C The representative images of HE staining and PSAT1 immunohistochemistry in late-stage and early-stage ccRCC pair tissues. The scale bars indicate 100 µm