Figure 4

Knockdown of NCAPD3 suppressed NSCLC cell proliferation by PI3K/AKT/FOXO4 signaling. a A volcano scatterplot illustrated the differentially expressed genes (DEGs). Blue dots show downregulated genes by NCAPD3 knockdown, grey dots represent unaltered genes, and red dots represent upregulated genes. (|Fold of changes| >1, p value < 0.05). b KEGG enrichment analysis was performed to investigate the downstream signaling pathway by identifying DEGs [19, 23]. c PI3K/AKT/FOXO4 components in A549 and SPCA-1 cells were assessed by Western blot. d Western blot was conducted to examine cell apoptosis-related markers (Bcl-2, Bax, and Caspase-8) and cell proliferation-related markers (Cyclin D1, Cdk4 and P27) in infected A549 and SPCA-1 cells treated with IGF-1 (100 ng/mL) for 24 h. (***p < 0.001, ****p < 0.0001)