Figure 2

Knockdown of NCAPD3 inhibited NSCLC cell proliferation and invasion. a-b The expression of NCAPD3 in NSCLC cell lines was assessed through RT-qPCR and Western blot. c-d The knockdown efficiency was detected through RT-qPCR and Western blot in A549 and SPCA-1 cells by transfecting with specific shRNAs. e The effect of NCAPD3 on cell proliferation was measured using CCK-8 assays. f The proliferation of cells was determined by EdU assays. g Transwell analysis of the invasive capacity of A549 and SPCA-1 cells. h Migration capacity was evaluate by wound-healing assays; all results were expressed as the means ± SD. (***p < 0.001; ****p < 0.0001)