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Table 5 RT-qPCR cycling conditions

From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient saliva

Stage

Cycling conditions

Denaturation

95 °C, 15 seconds

×  40 cycles

Annealing and elongation

60 °C, 1 minute

Hold

4 °C

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BMC Cancer

ISSN: 1471-2407

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