Skip to main content

Table 2 RT-qPCR components per 5 μL reaction

From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient saliva

Component

Volume – 1x reaction (μL)

TaqMan Universal PCR Master Mix (20X)

2.5

TaqMan Assay for E6/E7 (20X)

0.5

cDNA

1.0

Water

1.0

Total

5.0

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us