Table 1 cDNA synthesis components per 20 μL reaction
Component | Volume – 1x reaction (μL) |
|---|---|
10x Reverse Transcriptase Buffer 1.0 mL | 2.0 |
25x dNTP Mix 100 mM, 200 μL | 0.8 |
RNase Inhibitor 100 μL, 20 Units/μL | 1.0 |
RNA Input (various concentrations) | 1.0 |
10x RT Random Primer, 1.0ML | 2.0 |
MultiScribe™ Reverse Transcriptase 100 μL, 50 units/μL | 1.0 |
Nuclease-free water | 12.2 |
Total | 20.0 μL |