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Fig. 5 | BMC Cancer

Fig. 5

From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient saliva

Fig. 5

Biplex detection of E6 and E7 mRNA in saliva specimens. RNA was isolated from the saliva of eight HNSCC patients (n = 3 p16 positive; n = 5 p16 negative). Negative experimental controls included cDNA (NTC) and PCR negative reactions. All reactions were completed in triplicate. A Beta 2 Microglobulin (B2M) was used as a positive control and reference gene to ensure expression observed in the E6 and E7 assays was positive and accurate, B E6 assay, C E7 assay

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BMC Cancer

ISSN: 1471-2407

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