Fig. 3From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient salivaBiplex and singleplex detection of E6 and E7 mRNA in patient tissue. Controls included SiHa cells as a positive control. All reactions were completed in triplicate. AÂ Biplex E6 assay; BÂ Biplex E7 assay. CÂ Singleplex E6 DÂ Singleplex E7. EÂ PCR effieciencies for E6 and FÂ E7 in patient tissue specimensBack to article page