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Fig. 2 | BMC Cancer

Fig. 2

From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient saliva

Fig. 2

Evaluating total RNA input for E6 and E7 Single and Biplex reactions. A E6 plex assay; B E7 plex assay. Cq 35 was determined to be a reasonable threshold for a positive result. All reactions were completed in triplicate. PCR efficiencies for C E6 assay; D E7 at different total RNA inputs. 100% PCR efficiency is depicted at 2.0, with the acceptable threshold of efficiency at 1.5

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BMC Cancer

ISSN: 1471-2407

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