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Fig. 1 | BMC Cancer

Fig. 1

From: Biplex quantitative PCR to detect transcriptionally active human papillomavirus 16 from patient saliva

Fig. 1

Single and Biplex detection of E6/E7 mRNA in HPV16 positive cell lines. HPV16 positive cell lines included SiHa and Caski, while HPV16 negative cell lines included HeLa (HPV18 positive), and MCF-7 (breast cancer cell line). All reactions were completed in triplicate. A E6 plex assay; B E7 plex assay. C PCR efficiencies for E6. D PCR efficiencies for E7 assay. 100% PCR efficiency is depicted at 2.0, with the acceptable threshold of efficiency at 1.5

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BMC Cancer

ISSN: 1471-2407

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