Fig. 4

Antigen-exposed CD8 T cells were selectively activated and expanded through the Phyduxon-T manufacturing process in vitro. Cells from patients with OC were harvested on day 0 (fresh PBMCs) and day 12 (Phyduxon-T) and stained with monoclonal antibodies to acquire T cell phenotype via flow cytometry. a We evaluated the changes in CD4 and CD8 on T cells, (b) the expression levels of CCR7⁺CD45RO⁺(central memory) and CCR7^-CD45RO⁺(effector memory) on CD14⁻CD19⁻CD56⁻TCRγδ⁻CD4⁻CD45⁺TCRαβ⁺CD8⁺ cells, (c, d) the expression levels of CD25 and CD69 on CD14⁻CD19⁻CD56⁻TCRγδ⁻CD4⁻CD45⁺CD3⁺TCRαβ⁺CD8⁺cells, (e, f) the expression level of IFN-γ on CD14⁻CD19⁻CD56^-CD3⁺CD8⁺4-1BB⁺ cells. The results shown in (c) and (e) are representative of six independent experiments. Data are presented as means ± SD of six independent experiments. Differences between groups were analyzed using the nonparametric Mann-Whitney test. p values:*p < 0.05,**p < 0.01