Fig. 3

Phyduxon exhibited NK cell-dependent cytotoxicity and induced proliferation and AIM expression of CD8 T cells. a Freshly isolated and cultured OC cells were stained with monoclonal antibodies. The expression of cytokeratin-7/8 on CD45⁻ cells was evaluated through flow cytometry. b A triple-cell coculture assay was performed to determine the APC function of Phyduxon after engaging with primary OC cells. c, d OC cells were cocultured with Phyduxon, and the TFL-4⁺caspase/granzyme B⁺ pattern was analyzed through flow cytometry to quantify apoptosis. e, f Phyduxon was cocultured with or without OC cells to determine the expression levels of IFN-γ and 4-1BB on proliferated CD3⁺CD8⁺ T cells. The results shown in (c) and (e) are representative of six and five independent experiments, respectively. Data in (a) and (d) are presented as means ± SD of six independent experiments, while data in (f) are presented as means ± SD of five independent experiments. Differences between groups were analyzed using the nonparametric Mann-Whitney test. p values: * p < 0.05, ** p < 0.01