Fig. 2

Phyduxon, as a human IKDC counterpart, exhibited the phenotypes and activities of DCs. a, b Comparison of the expression levels of HLA-DR, CD86, and CD11c on CD3⁻CD14⁻CD19⁻CD45⁺CD56⁺cells between days 0 and 12 through flow cytometry. c, d Phyduxon was isolated, and its allostimulatory APC function was evaluated by coculturing with allogeneic CFSE-labeled CD25⁻-responder cells (Res.). Responder CD3 T-cell proliferation was assessed by determining the pattern of CFSE dilution through flow cytometry. The results shown in (a) and (c) are representative of six and four independent experiments, respectively. Data in (b) and (d) are presented as means ± SD of six and four independent experiments, respectively. Differences between groups were analyzed using the nonparametric Mann-Whitney test. p values:*p < 0.05,**p < 0.01