Fig. 1

Phyduxon, as a human IKDC counterpart, exhibited the phenotypes and activities of NK cells. a Schematic of the manufacturing process for Phyduxon-T. PBMCs were cultured in AIM-V medium plus HPL, containing hIL-15 (days 0, 3, 6, and 9), hIL-12 (days 0, 3, 6, and 9), and hIL-18 (days 0 and 3) for 12 days to generate Phyduxon-T. b, c Comparison of the expression of CD16 and NKG2D on CD3⁻CD14⁻CD19⁻CD45⁺CD56⁺cells between days 0 and 12 through flow cytometry. d, e Phyduxon was isolated and evaluated for its cytotoxicity after coculturing with K562 tumor cells, analyzing the TFL-4⁺caspase/granzyme B⁺ pattern through flow cytometry. The results shown in (b) and (d) are representative of six independent experiments. Data in (c) and (e) are presented as means ± SD of six independent experiments. Differences between groups were analyzed using the nonparametric Mann-Whitney test. p values: **p < 0.01; ns, not significant