Fig. 3
Effect of NR2F1 overexpression on the dormancy, migration and invasion of SACC-83 and SACC-LM cells. (a)CCK8 assay was used to examine the cell growth rates in control and NR2F1hi SACC cells group. The data showed that the cell growth rates were significantly suppressed in NR2F1hi SACC cells. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (b) Flow cytometry was used to examine the cell cycle in control and NR2F1hi SACC cells group. Representative figures of three independent experiments were shown. Compared with the control, there were more NR2F1high SACC cells in G0/G1 phases and less cells in G2/M phases (p < 0.05). (c) Flow cytometry showed cell apoptosis in control and NR2F1hi SACC cells group. Apoptotic analysis of SACC cells showed no difference between control and NR2F1hi SACC cells group (p > 0.05). Representative figures of three independent experiments were shown. (d) Migration assay examined the cell migration ability in control and NR2F1hi SACC cells group. Representative figures of three independent experiments were shown. NR2F1 high expression could promote the migration ability of SACC cells. The mean was derived from cell counts of 3 fields, and each experiment was repeated 3 times. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (e).Invasion assay examined the cell invasive ability in control and NR2F1hi SACC cells group. Representative figures of three independent experiments were shown. NR2F1 high expression could promote the invasion ability of SACC cells. The mean was derived from cell counts of 3 fields, and each experiment was repeated 3 times. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05