Fig. 2

NR2F1 overexpression via lentivirus transfection in SACC cells. (A) Immunofluorescence staining of NR2F1 in NR2F1- and vector- transfected SACC cells, where blue represented staining for DAPI and green represented staining for NR2F1. Scale bar = 20 μm, SP × 200. (b) Western blot showed that the protein level of NR2F1 was overexpressed in NR2F1 transfected SACC-83 and SACC-LM, absent in vector groups. Lamin B was identified as control reference. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (c) RT-PCR assay showed that the mRNA level of NR2F1 in SACC-83 and SACC-LM was significantly rise in NR2F1 transfected groups and could not be detected at vector counterparts. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05