Fig. 5

TRIM28 protects MAGEC2 from proteasomal degradation. a, b Downregulation of TRIM28 promotes the proteasome-mediated degradation of MAGEC2 in A375 cells (a) or Hs 695 T cells (b). TRIM28 specific siRNAs or si-NC were transfected into Hs695 T or A375 cells. Twenty-four hours after transfection, cells were treated with PS-341 (10 μM), MG132 (20 μM) or vehicle (DMSO) for 8 h before immunoblotting with indicated antibodies. c Lysosome inhibitor does not affect the level of MAGEC2 protein in TRIM28 knockdown Hs 695 T cells or A375 cells. TRIM28 specific siRNAs or si-NC was transfected into A375 (left panel) or Hs695 (right panel) T cells. Twenty-four hours after transfection, cells were treated with CQ (20 μM) or vehicle (DMSO) for 24 h before immunoblotting with indicated antibodies. Detection of p62 is served as a positive control for treatment with CQ. Each experiment was repeated at least three times