Table 1 Bisulfite sequencing PCR primers and PCR conditions for BAS samples
Gene | Sequences (5′ to 3′) | Product size (bp) | Annealing (°C) | Annealing Time (s) | Position from TSS | UCSC gene ID |
|---|---|---|---|---|---|---|
CR2 | F: GGGTGAGTTTGAGTTAAAGAGTGG R: AAAAAACCAATAAAAACAATCAAAACCAAA | 514 | 58 | 50 | −149 – +365 | uc001hfv.3 |
ITGA4 | F: TGTAATTTTGGGGTAGTGGT R: CCCTCCTACCTCCTTAAAAAAAAAAAA | 362 | 58 | 45 | +711 − +1072 | uc002unu.3 |
RERG | F: GGAGTTTGGAGGTTTGGAAAT R: CAAAAACAAATACCAATAACCC | 278 | 58 | 45 | −145 – +133 | uc001rct.3 |
RRAD | F: TTGGTGGGGGTGGATAGATA R-CCTCCCCCAACCCCCAAAT | 331 | 61 | 45 | −102 – +228 | uc002eqo.2 |
SHISA3 | F: GGTTGAGAGTTAAGTTTTGGGGG R: CCTCCCCACTCCTCAAAAAAA | 446 | 58 | 45 | −570 – −125 | uc003gwp.3 |
ZNF549 | F: TTTTAGTTTGATGGGTTTTTTTTTTTGTT R: AAACCTCAAAACCCAAATAAAAATC | 502 | 56 | 45 | −177 – +325 | uc002qpb.2 |
ZNF671 | F: ATTTTGTTTTTGTTAGGTTGTTTTTGG R: CTATCCTAAAACACAAAAACTACAAACACT | 311 | 57 | 45 | +13 − +323 | uc002qpz.4 |