Table 1 The primer sequences of glycolytic enzyme

Jumonji domain containing 2A

JMJD2A

Sense: 5′-ATCCCAGTGCTAGGATAATGACC-3′

Anti-sense: 5′-ACTCTTTTGGAGGAACCCTTG-3′

Glucose transporter-1

GLUT-1

Sense: 5′-CTTTGTGGCCTTCTTTGAAGT-3′

Anti-sense: 5′-CCACACAGTTGCTCCACAT-3′

Glucose transporter-4

GLUT-4

Sense: 5′-CTTCATCATTGGCATGGGTTT-3′

Anti-sense: 5′-CGGGTTTCAGGCACTTTTAGG-3′

Hexokinase-II

HK-II

Sense: 5′-GATTTCACCAAGCGTGGACT-3’

Anti-sense: 5′-CCACACCCACTGTCACTTTG-3′

Glucose-6-phosphate isomerase

G6PI

Sense: 5′-AGGCTGCTGCCACATAAGGT-3′

Anti-sense: 5′-AGCGTCGTGAGAGGTCACTTG-3′

Muscle-type phosphofructokinase

PFK-M

Sense: 5′-ATTCGGGCTGTGTTCTGG-3′

Anti-sense: 5′-TGGCTAGGATTTTGAGGATGG-3′

Liver-type phosphofructokinase

PFK-L

Sense: 5′-GGACAGGAAAGAGGAAGTGAC-3′

Anti-sense: 5′-CGTAGATGAGGAAGACTTTGGC-3′

Platelet isoform of phosphofructokinase

PFK-P

Sense: 5′-CATCGACAATGATTTCTGCGG-3′

Anti-sense: 5′-CCATCACCTCCAGAACGAAG-3′

Aldolase B

AldoB

Sense: 5′-ATGCCACTCTCAACCTCAATGCTATC-3′

Anti-sense: 5′-TTATTTTCTTGGGTGGGTATTCTGG-3′

Phosphoglycerate kinase 1

PGK-1

Sense: 5′ -CGGTAGTCCTTATGAGCC-3′

Anti-sense: 5′-CATGAAAGCGGAGGTTCT-3′

Phosphoglycerate mutase 1

PGAM-1

Sense: 5′-CCTGGAGAACCGCTTC-3′

Anti-sense: 5′-CATGGGCTGCAATCAGTACAC-3′

Enolase

Enolase

Sense: 5′-CTGATGCTGGAGTTGGATGG-3′

Anti-sense: 5′-CCATTGATCACGTTGAAGGC-3′

M1 isoform of pyruvate kinase

PKM1

Sense: 5′-CTATCCTCTGGAGGCTGTGC-3′

Anti-sense: 5′-CCATGAGGTCTGTGGAGTGA-3′

M2 isoform of pyruvate kinase

PKM2

Sense: 5′-GGGTTCGGAGGTTTGATG-3′

Anti-sense: 5′-ACGGCGGTGGCTTCTGT-3’

Lactate dehydrogenase B

LDHB

Sense: 5′-CCTAGAGCTCACTAGTCACAG-3′

Anti-sense: 5′-CTCCTGTGCAAAATGGCAAC-3′

Lactate dehydrogenase A

LDHA

Sense: 5′-CAGCTTGGAGTTTGCAGTTAC-3′

Anti-sense: 5′-TGATGGATCTCCAACATGG-3′

Glyceraldehyde-3-phosphate dehydrogenase

GAPDH

Sense: 5′-TGACGCTGGGGCTGGCATTG-3′

Anti-sense: 5′-GCTCTTGCTGGGGCTGGTGG-3′