Fig. 5

Promoter DNA methylation alteration is inversely correlated to gene expression changes in DIP2C ^−/− edited cells. a Distribution of assayed sites in relation to CpG islands. Shore - 0–2 kb from CpG island, Shelf - 2–4 kb from CpG island, Open sea - isolated CpGs in the genome. b Distribution of assayed sites in relation to gene context. TSS1500 – 1500 bp upstream the transcription start site (TSS), TSS200 – 200 bp upstream the TSS, UTR – untranslated region, 1st exon –exon 1 of gene transcript. In (a) and (b) the distribution of all sites with methylation data recorded in the assay is given as reference to the distribution of the differentially methylated sites (hypomethylated and hypermethylated sites with beta diff ≥│0.3│). The fraction of sites relative to the respective total number of sites is indicated for each category. c-d Plots of the change in DNA methylation (beta diff) at promoter regions (c) and gene bodies (d) and change in expression for the respective gene. Black – genes with beta diff ≥│0.3│ (promoter n = 72, gene body n = 74); grey – genes with beta diff <│0.3│. Promoter region includes TSS1500, TSS200, 5′ UTR and 1st exon