Fig. 5

Promoter region of miR-200c were hypermethylated in TNBC cells. a, An online CpG island predictor was used to detect the CpG islands of 3000 bp of miR-200c promoter sequence, upstream of the miR-200c cluster. b, Immunofluorescence analysis showed the expression of E-cadherin (red), Vimentin (green) in MDA-MB-231 cells treated with or without 5aza for 72 h. Image were photographed by electron microscope at high magnification(×40). c, Migration ability of MDA-MB-231 cells were subjected to migration assay, the numbers of migration cells were represented by the mean of three individual experiments. Data were expressed as mean ± SD (n = 3), * P < 0.05; d, Relative expressions of miR-200c cells in MDA-MB-231 cells treated with or without 5aza for 72 h determined by realtime -PCR. miRNA expressions were normalized to small nuclear ribonucleic acid (snRNA) RNU6B, and results were expressed as fold change relative to control (con). e, Western blot analysis showed the expression of E-cadherin and Vimentin in MDA-MB-231 cells treated with or without 5aza for 72 h. All experiments were repeated 3 times. Results were expressed as mean ± SD (n = 3). * P < 0.05