Fig. 4

ALKBH3 promoter methylation in primary breast tumors and adjacent normal breast tissue. a Differential CpG methylation over the ALKBH3 promoter is observed in a subset of primary breast tumors. The tumor (T; red coloured bars) and normal breast tissue (N; grey coloured bars) samples are matched, i.e. derived from the same individual and arranged side-by-side on the x-axis with ALKBH3 promoter methylation for each sample represented as a bar. The standard deviation is shown (line extensions from the bars). The P-value shown was derived from a paired Wilcoxon’s hypothesis test (P = 0.00012). b Box and whisker plot of ALKBH3 mRNA expression in normal breast tissue samples and primary breast tumors. The P-value shown was derived from a Wilcoxon’s hypothesis test (P = 0.014). c ALKBH3 promoter methylation data plotted on x-axis and mRNA expression data on y-axis for primary breast tumors. The P-value shown was derived from Spearman’s correlation testing (Spearman’s rho = −0.577; P = 0.024)