Fig. 3
From: Regulation of early growth response 2 expression by secreted frizzled related protein 1
Loss of SFRP1 increases TGF-β mediated ERK1/2 activation in MMECs. a Control and Sfrp1 −/− MMECs were treated with 2.5 ng/ml TGF-β1 and/or 10 μM LY364947 in triplicate wells and cell lysates were analyzed for phospho-ERK1/2 and β-Actin protein expression by western blot. a Image of representative western blot illustrating band densities in response to treatment and b quantification of integrated band densities from three separate western blots. *p < 0.05, (significantly different from vehicle treated cells using a student’s t-test)