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Fig. 5 | BMC Cancer

Fig. 5

From: Identifying global expression patterns and key regulators in epithelial to mesenchymal transition through multi-study integration

Fig. 5

C1orf116: a novel EMT regulator. a qPCR: mRNA expression of C1orf116 in EMT model prostate cancer cell lines PC3-Epi, PC3-EMT and PC3-TxR * P < 0.1; ** P < 0.05; *** P < 0.005. b Immunoblot: Protein expression of C1orf116 in EMT model prostate cancer cell lines PC3-Epi, PC3-EMT and PC3-TxR (LSR, DPYSL3, S100A14, C1orf116, and β-actin were all probed on the same blot, so the β-actin loading control is appropriate for both Fig. 4c (LSR, DPYSL2, S100A14) and Fig. 5b (C1orf116). Data were separated into two figures for clarity). c qPCR: mRNA expression of C1orf116 and other known epithelial (OVOL1, ESRP1 and CDH1) and mesenchymal (CDH2) gene in PC3-Epi cells transfected with C1orf116-siRNA relative to empty vector control * P < 0.1; ** P < 0.05; *** P < 0.005

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BMC Cancer

ISSN: 1471-2407

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