Fig. 5
From: Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer
GGT1 activity and GGT1 expression in exosomes isolated from human serum by differential centrifugation. a Exosomes isolated from serum of a healthy individual (500 μL) by differential centrifugation were separated by OptiPrep density gradient centrifugation. After ultracentrifugation, fractions were subjected to Western blot analysis for GGT1 large and small subunits and CD9. The upper band of the doublet corresponds to the GGT1 small subunit (shown by arrow). b Serum exosomes isolated by differential centrifugation from BPH (n = 4) and PC (n = 8) patients were subjected to Western blot analysis for GGT1 large and small subunits and CD9 as well as measurement of GGT activity using gGlu-HMRG. c Spearman’s rank correlation coefficient analysis was performed between the signal intensity of GGT1 large subunit and GGT activity