Cells | Time (hrs) | Treatment | Cell cycle distribution (%)(Average ± SE) | |||
---|---|---|---|---|---|---|
Sub-G1 | G1 | S | G2/M | |||
MSTO-211H | 24 | (-) | 4.35 ± 0.22 | 56.30 ± 0.35 | 17.93 ± 0.04 | 20.88 ± 0.44 |
 | Met 20mM | 5.43 ± 0.23 | 68.82 ± 0.25 | 12.91 ± 0.22 | 12.39 ± 0.23 | |
 | Met 60 mM | 10.92 ± 0.63 | 63.25 ± 1.75 | 4.71 ± 0.16 | 20.88 ± 2.13 | |
 | Nut 10 μM | 5.95 ± 0.11 | 84.18 ± 0.43 | 2.35 ± 0.16 | 7.32 ± 0.23 | |
 | Met 20mM + Nut 10 μM | 7.39 ± 0.14 | 72.91 ± 0.29 | 3.00 ± 0.14 | 16.23 ± 0.29 | |
 | Met 60 mM + Nut 10 μM | 10.56 ± 1.42 | 61.41 ± 1.50 | 7.93 ± 0.69 | 19.71 ± 0.71 | |
48 | (-) | 0.76 ± 0.03 | 68.87 ± 0.65 | 12.70 ± 0.59 | 17.31 ± 0.20 | |
 | Met 20mM | 3.40 ± 0.06 | 70.64 ± 0.26 | 11.96 ± 0.06 | 13.43 ± 0.21 | |
 | Met 60 mM | 7.44 ± 0.12* | 68.10 ± 0.30 | 5.29 ± 0.08 | 18.84 ± 0.13 | |
 | Nut 10 μM | 6.11 ± 0.22* | 85.90 ± 0.25 | 1.89 ± 0.03 | 5.92 ± 0.13 | |
 | Met 20mM + Nut 10 μM | 6.51 ± 0.16 | 76.44 ± 0.24 | 2.82 ± 0.08 | 13.87 ± 0.18 | |
 | Met 60 mM + Nut 10 μM | 11.34 ± 0.22* | 63.25 ± 0.24 | 2.97 ± 0.04 | 22.25 ± 0.22 | |
72 | (-) | 1.15 ± 0.11 | 82.29 ± 0.39 | 4.04 ± 0.17 | 12.26 ± 0.18 | |
 | Met 20mM | 3.08 ± 0.09 | 76.19 ± 0.21 | 8.07 ± 0.14 | 12.25 ± 0.42 | |
 | Met 60 mM | 13.28 ± 0.27* | 65.29 ± 1.07 | 4.99 ± 0.10 | 16.24 ± 0.71 | |
 | Nut 10 μM | 11.38 ± 0.14* | 81.30 ± 0.28 | 1.88 ± 0.10 | 5.29 ± 0.13 | |
 | Met 20mM + Nut 10 μM | 7.39 ± 0.15 | 75.38 ± 0.21 | 2.63 ± 0.06 | 14.26 ± 0.23 | |
 | Met 60 mM + Nut 10 μM | 36.25 ± 0.44* | 44.02 ± 0.25 | 3.28 ± 0.06 | 16.15 ± 0.26 | |
NCI-H28 | 24 | (-) | 0.47 ± 0.04 | 60.27 ± 0.19 | 16.66 ± 0.34 | 22.06 ± 0.25 |
 | Met 20mM | 0.43 ± 0.02 | 67.39 ± 0.41 | 11.76 ± 0.29 | 19.66 ± 0.13 | |
 | Met 60 mM | 0.89 ± 0.08 | 44.81 ± 0.07 | 15.01 ± 0.17 | 38.33 ± 0.17 | |
 | Nut 10 μM | 0.77 ± 0.02 | 40.63 ± 0.20 | 9.25 ± 0.42 | 47.61 ± 0.36 | |
 | Nut 40 μM | 1.22 ± 0.07 | 44.87 ± 0.57 | 15.17 ± 0.57 | 37.12 ± 0.65 | |
 | Met 20mM + Nut 10 μM | 1.25 ± 0.04 | 42.33 ± 0.36 | 13.30 ± 0.38 | 41.28 ± 0.06 | |
 | Met 60 mM + Nut 40 μM | 1.24 ± 0.15 | 48.20 ± 0.37 | 20.92 ± 0.17 | 28.35 ± 0.38 | |
48 | (-) | 1.54 ± 0.05 | 60.08 ± 0.39 | 16.04 ± 0.36 | 21.88 ± 0.01 | |
 | Met 20mM | 0.45 ± 0.04 | 73.97 ± 0.18 | 6.35 ± 0.05 | 18.66 ± 0.10 | |
 | Met 60 mM | 1.06 ± 0.10 | 40.68 ± 0.08 | 14.65 ± 0.19 | 42.61 ± 0.19 | |
 | Nut 10 μM | 3.84 ± 0.10 | 43.82 ± 0.29 | 8.28 ± 0.20 | 42.47 ± 0.26 | |
 | Nut 40 μM | 2.50 ± 0.10 | 49.77 ± 0.22 | 10.35 ± 0.10 | 36.08 ± 0.21 | |
 | Met 20mM + Nut 10 μM | 0.91 ± 0.09 | 46.45 ± 0.74 | 9.23 ± 0.29 | 41.90 ± 0.90 | |
 | Met 60 mM + Nut 40 μM | 1.38 ± 0.12 | 49.24 ± 0.21 | 20.16 ± 0.30 | 27.63 ± 0.15 | |
72 | (-) | 1.49 ± 0.02 | 64.68 ± 0.34 | 15.58 ± 0.03 | 17.79 ± 0.30 | |
 | Met 20mM | 0.52 ± 0.09 | 75.59 ± 0.14 | 4.52 ± 0.08 | 18.86 ± 0.21 | |
 | Met 60 mM | 1.50 ± 0.31 | 41.66 ± 0.37 | 15.31 ± 0.03 | 40.59 ± 0.27 | |
 | Nut 10 μM | 6.09 ± 0.20 | 43.98 ± 0.25 | 8.75 ± 0.23 | 39.57 ± 0.41 | |
 | Nut 40 μM | 8.96 ± 0.83 | 61.92 ± 0.79 | 7.31 ± 0.02 | 21.05 ± 0.49 | |
 | Met 20mM + Nut 10 μM | 1.01 ± 0.28 | 46.14 ± 0.66 | 9.14 ± 0.11 | 42.20 ± 0.41 | |
 | Met 60 mM + Nut 40 μM | 9.00 ± 0.72 | 44.61 ± 0.43 | 15.83 ± 0.25 | 29.48 ± 0.45 | |
EHMES-10 | 24 | (-) | 0.46 ± 0.05 | 70.86 ± 0.69 | 9.64 ± 0.3 | 18.63 ± 0.57 |
 | Met 20mM | 1.04 ± 0.06 | 73.58 ± 0.31 | 8.78 ± 0.05 | 16.18 ± 0.32 | |
 | Met 60 mM | 1.61 ± 0.06 | 67.93 ± 0.31 | 6.95 ± 0.14 | 23.13 ± 0.28 | |
 | Nut 20 μM | 1.19 ± 0.10 | 73.47 ± 0.21 | 10.38 ± 0.26 | 14.38 ± 0.29 | |
 | Nut 60 μM | 1.19 ± 0.08 | 80.17 ± 0.16 | 3.49 ± 0.06 | 14.74 ± 0.05 | |
 | Met 20mM + Nut 20 μM | 0.85 ± 0.02 | 76.79 ± 0.23 | 7.35 ± 0.09 | 14.61 ± 0.19 | |
 | Met 60 mM + Nut 60 μM | 2.37 ± 0.19 | 73.06 ± 0.38 | 5.2 ± 0.38 | 18.93 ± 0.32 | |
48 | (-) | 0.92 ± 0.04 | 73.14 ± 0.06 | 10.34 ± 0.23 | 15.22 ± 0.35 | |
 | Met 20mM | 1.46 ± 0.13 | 61.87 ± 0.16 | 13.00 ± 0.12 | 23.04 ± 0.21 | |
 | Met 60 mM | 2.67 ± 0.12* | 66.72 ± 0.12 | 9.32 ± 0.11 | 20.73 ± 0.13 | |
 | Nut 20 μM | 1.62 ± 0.20 | 67.78 ± 0.21 | 12.12 ± 0.17 | 18.02 ± 0.17 | |
 | Nut 60 μM | 1.85 ± 0.16* | 84.44 ± 0.12 | 3.32 ± 0.05 | 10.15 ± 0.06 | |
 | Met 20mM + Nut 20 μM | 1.35 ± 0.04 | 66.71 ± 0.35 | 13.01 ± 0.32 | 18.19 ± 0.42 | |
 | Met 60 mM + Nut 60 μM | 4.57 ± 0.17* | 74.63 ± 0.17 | 5.44 ± 0.16 | 15.11 ± 0.2 | |
72 | (-) | 2.18 ± 0.55 | 56.86 ± 0.62 | 12.2 ± 0.75 | 27.79 ± 1.57 | |
 | Met 20mM | 1.89 ± 0.11 | 63.77 ± 0.19 | 11.90 ± 0.21 | 21.64 ± 0.42 | |
 | Met 60 mM | 3.07 ± 0.14* | 64.22 ± 0.13 | 8.22 ± 0.12 | 23.99 ± 0.22 | |
 | Nut 20 μM | 0.73 ± 0.03 | 67.46 ± 0.28 | 11.05 ± 0.19 | 20.22 ± 0.39 | |
 | Nut 60 μM | 3.47 ± 0.11* | 84.27 ± 0.25 | 3.4 ± 0.17 | 8.74 ± 0.07 | |
 | Met 20mM + Nut 20 μM | 1.27 ± 0.03 | 70.85 ± 0.23 | 9.44 ± 0.43 | 17.85 ± 0.19 | |
 | Met 60 mM + Nut 60 μM | 13.85 ± 0.86* | 62.12 ± 0.26 | 6.35 ± 0.25 | 17.57 ± 0.86 |