Fig. 4

Involvement of p53 in metformin- and nutlin-3a-mediated cytotoxicity. a Western blot analysis to analyze p53 down-regulation. MSTO-211H cells treated with either p53-siRNA or control siRNA were incubated with nutlin-2a for 24 h. GAPDH expression was used as a loading control. b Cells were treated with p53-siRNA or control siRNA, and susceptibility to metformin or nutlin-3a was examined with the WST assay. Relative viability was calculated based on untreated cells. Averages and SE bars are shown (n=3)