Fig. 3
LPA receptor expression in pancreatic cancer cell lines. a mRNA expression in 4 pancreatic cancer cell lines determined by quantitative RT-PCR. The relative expression of each receptor was calculated based on the LPA1 expression in BxPC3. Data represent mean values of 3 independent experiments. The error bars represent the standard error. Statistical analysis was conducted using the Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001 (vs. BxPC3). b Protein expression in 4 pancreatic cancer cell lines detected by SDS-PAGE and western blotting. A prostate cancer cell line, PC3, a gastric cancer cell line, KATOIII, and a pleural mesothelioma cell line, 211H, were used as positive controls. β-actin was used as a loading control and its expression is also shown. The arrow-head indicates the specific bands of each LPA receptor. M, protein marker; Mia, MIAPaCa-2; Photographs are representative of 3 independent experiments. The intensity of each band was measured and the relative expression of each receptor protein was calculated based on the receptor in BxPC3 cells. Quantitative data represent mean values of 3 independent experiments except the positive controls PC3 and 211H. The error bars represent the standard error