Fig. 4

OPCML arrested cell cycle and induced apoptosis of gastric cancer cells. a1 and b1 Representative images of cell cycle distribution of SGC-7901 (a1) and BGC-823 (b1) cells. a2 and b2 Statistical analysis of the distribution percentage of cells in G0/G1, S, G2/M phases of SGC-7901 (a2) and BGC-823 (B2) cells. c1 and d1 Representative images of apoptosis of SGC-7901 (c1) and BGC-823 (d1) cells. c2 and d2 Statistical analysis of early apoptosis and late apoptosis ratio of SGC-7901 (c2) and BGC-823 (d2) cells. (Data are mean ± SE, versus empty vector; n = 5 independent experiments in triplicate). e changes of protein expression of G1/S phase transition regulator and the active form of pro-apoptosis regulators, as well as the phosphorylation levels of AKT and GSK3β in SGC-7901 and BGC-823 cells. ** P < 0.01,* P < 0.05