Fig. 3

RhoBTB1 regulates Golgi integrity through METTL7 proteins. a-b HeLa cells were transfected with siRNAs targeting METTL7A, METTLB or both. After 48 h, RNA was prepared from the cells and the expression of each METTL7 was quantified by RT-PCR. Data are means ± SEM (n = 3). c HeLa cells were transfected with the same siRNAs and fixed and stained for the Golgi marker giantin. Silencing of either methyltransferase caused a marked and significant fragmentation of the Golgi. Data are means ± SEM (n = 3). Comparisons are to the control lamin siRNA. d Shows representative images of the cells. Golgi are stained in green; nuclei in blue. e HeLa cells were transfected with siRNAs targeting RhoBTB1 and with plasmids expressing myc-tagged METTL7A or METTL7B. After 48 h, the cells were fixed and stained for the Golgi marker giantin (green) and for the transfected methyltransferases (red). Bars = 10 μm. f Quantification of transfected cells showed that expression of either METTL7 rescued Golgi morphology in cells depleted of RhoBTB1. Data are means ± SEM (n = 3); comparisons are as indicated. *P <0.05; **P <0.01, ***P <0.001, ****P <0.0001