Testis specific Y-like 5: gene expression, methylation and implications for drug sensitivity in prostate carcinoma

Table 1 Summary of TSPYL5 mRNA expression, DNA methylation frequency, protein expression based on Gleason score

Pathology	Age (yrs)	GS	No of samples	mRNA^a expression	Methylation^b frequency (%)	IHC^c
Normal	40–60	----	3	3/3	<10	-
Benign	59–79	----	9	7/9	25	-
Adenocarcinoma	36–50	6	4	3/4	28	12
Adenocarcinoma	50–70	7	14	11/14	39	8
Adenocarcinoma	60–68	≥8	3	0/3	70	0

^aPCR analysis of TSPYL5 mRNA expression in benign and prostate adenocarcinoma
^bAnalysis of DNA methylation (MSP) in the tissues (n = 3 each). The gel band intensities were quantified by Image J software as noted in methods
^cComposite score (CS) is derived from staining intensity times percent positive cells for TSPYL5 protein expression

ISSN: 1471-2407