Fig. 13

AZ and/or MS-275 treatments reduced the expression of stem cell markers in NB xenografts. a-b present IHC staining (x20 and x40) for OCT4 cell localization and number of OCT4 positive cells, c-d SOX2 cell localization and number of SOX2 positive cells, and e-f Nanog cell localization and number of Nanog positive cells after 14 days treatment with AZ, MS-275 and AZ + MS-275 compared to untreated group in SH-SY5Y xenografts. The number of OCT4 positive cells was reduced after treatment with AZ by 37 ± 0.35% (p < 0.05), MS-275 by 63 ± 0.85% (p < 0.001) and AZ + MS-275 by 82 ± 0.45% (p < 0.001). The number of SOX2 positive cells was reduced in AZ by 32 ± 0.60% (p = 0.01), MS-275 by 61 ± 0.5% (p = 0.0009) and AZ + MS-275 by 82 ± 0.46% (p = 0.0002). The number of Nanog positive cells was reduced in AZ by 11 ± 0.60% (p > 0.05), MS-275 by 54 ± 0.45% (p = 0.0005) and AZ + MS-275 by 70 ± 0.76% (p = 0.0002)