Fig. 3
From: Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16
The promoter of HPV 16 E6 contains methylation sites with five CpGs dinucleotides located at 31Â nt, 37Â nt, 43Â nt, 52Â nt and 58Â nt. After genomic DNA is treated with sodium bisulphate, T represents unmethylated C and C represents methylated C. The proportion of C represents the methylation frequency in the CpG dinucleotides. In this representative sequence, the methylation frequencies of the five CpGs at 31Â nt, 37Â nt, 43Â nt, 52Â nt and 58Â nt were 20, 16, 14, 20, and 19%, respectively