Fig. 3

PD0325901 increases resistance to shear-stress induced detachment, decreases cell motility, and reduces aggregate dispersal velocity. Untreated and PD0325901-treated GBM cells attached to PET membranes were subjected to 30 dynes/cm of shear flow for 3 h, whereupon the number of cells retained on the membranes was quantified. For GBM-1, GBM-2 and GBM-4, PD0325901 treatment resulted in a significant retention of cells (a). A fluorescent microbead phagokinetic track assay was used to measure cell motility. For all GBM lines, PD0325901 significantly decreased cleared area. Co-incubation of cells with PD0325901 and 5 μg/ml anti-human fibronectin antibody, hFN 7.1, restored motility to control levels. Asterisks represent statistical difference using ANOVA, p < 0.0001, and Tukey’s multiple comparisons tests (b). The dispersal velocities of PD0325901-treated aggregates (n = 24) was significantly lower than those measured for PD0325901-treated aggregates (n = 24, c). For Fig. 3a and c, asterisks represent significant difference at p < 0.05 by pair-wise comparison using Student t-test