Fig. 2
From: Inhibition of glioblastoma dispersal by the MEK inhibitor PD0325901
Assessment of aggregate cohesion, stiffness, and viscosity in response to PD0325901 treatment. Surface tension measurements for GBM-1-4 aggregates generated using standard TCM and treated with either DMSO or PD0325901. n = 20, pair-wise comparison by Student t-test, p > 0.05 (a). Surface tension measurements of GBM-3 aggregates generated in fibronectin-depleted medium supplemented with 300 μg/ml of human fibronectin. n = 20, pair-wise comparison by Student t-test, p < 0.0001 (b). Stiffness (c) and viscosity (d) data for GBM-3 aggregates generated in fibronectin-depleted medium supplemented with either 30 μg/ml or 300 μg/ml human fibronectin. Asterisks represent statistical significance by pair-wise Student t-test, p < 0.05. Note significant increase in stiffness and viscosity in response to increased concentrations of fibronectin