Fig. 4

Kinetic of serum IgG antibody responses against P467. The levels of serum IgG antibody responses, induced 2, 4 and 6 months after the last immunization with different amounts of P467-CRM conjugate administered with Alum or Montanide, are shown. * The mice in this group were sacrificed before the six-month period, and their splenocytes were used for the T cell epitope mapping analysis (Table 2). The OD values are after subtraction of the background (i.e. pre-immunized mice), and based on ELISA experiments as described in materials and methods. The statistical analysis was performed using Generalized Estimation Equations. Significant difference is denoted by ***