Fig. 2

Expression of Somatostatin and its receptors in HT29 and SW480 colon cancer cell lines and patient matched normal and tumor tissue samples. a Cells were grown to 70–80% confluency, RNA was isolated and RT-PCR was performed. The HT29 cells express SST, SSTR1, SSTR2, and SSTR4. The SW480 cells express faint SSTR1, SSTR2, SSTR3, and SSTR4. This analysis was repeated three times and the gel images are representative of one data set for each cell line. b RT-PCR was performed to look at mRNA levels of SST and SSTR1-5 in these 5 patient samples. In all the normal samples, SST and SSTR1 were expressed. However, in the matching tumor samples, SST expression was gone and SSTR1 was present in 4 out of 5samples. N1 and T1 = matched normal and tumor tissue of patient #1. c Sorted ALDH+ and ALDH- cells from SW480 and HT29 cell lines were analyzed for mRNA expression of somatostatin and its receptors. RT-PCR analysis was performed on ALDH- and ALDH+ cells. These results indicate that the ALDH+ cells do not co-express somatostatin or its receptors in either cell line