Fig. 5
Immunoblots depicting the level of LC3, LAMP1, Beclin 1, Bcl-2, p62 and Atg7 proteins in MCF-7DOX2 cells at selection doses 7 through 12 (7–12) grown in the presence of bafilomycin A1. Immunoblots also depict the level of these proteins in MCF-7CC10 cells (cc), grown in the absence of bafilomycin A1. Bafilomycin (50 nM) was added to block degradation of the proteins by lysosomal proteases. For control MCF-7CC10 cells (cc, lane1), a volume of ethanol equal to that of added bafilomycin A1 was used as a vehicle control. All images are representative of at least three independent experiments. Molecular weight standards are depicted in lane M