Fig. 2From: Role of autophagy and lysosomal drug sequestration in acquired resistance to doxorubicin in MCF-7 cellsDistribution of lysosomes and doxorubicin in MCF-7CC10 and MCF-7DOX2–10 cells. Lysosomes in cells were visualized by laser scanning confocal microscopy after labelling with LysoTracker® and appeared green in colour. Doxorubicin distribution is depicted in red due to its autofluorescent nature. The images in this figure are representative of approximately 100 cells viewed on two separately stained slides in two independent experiments. Each image represents at least 10 microscopic photos taken from the two experiments. The localization of lysosomes and doxorubicin was also confirmed in three dimensions using stacked images obtained by confocal microscopy (data not shown) in two replicate experimentsBack to article page