Fig. 4From: CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL)TUNEL assay to detect apoptosis with HAS2, CD44 or RHAMM loss in bladder cancer cells +/− AGL. a, b UMUC3 and T24T shCTL and shAGL cells were plated in chambered slides and 24 h later transfected with scrambled siRNA or siRNA against HAS2 (siHAS2), CD44 (siCD44) or RHAMM (siRHAMM). Details of siRNA are in Material and Methods. 48 h after transfection cells were subjected to TUNEL assay according to manufacturer protocol as mentioned in Material and Methods. Images were taken at a 40X magnification using Olympus IX71 microscope. c, d Quantification of TUNEL staining in UMUC3 and T24T shCTL and shAGL cells with the different gene knockdowns (n = 3). TUNEL staining quantified using ImageJ. Results are shown as mean ± SD, *P < 0.05Back to article page