From: Changes in the in vitro activity of platinum drugs when administered in two aliquots
Spot | ID | Full name | MALDI | location | Expression in A2780cisR | Post treatment expression | Function | Tumour association | ||
---|---|---|---|---|---|---|---|---|---|---|
CS | CS + CS | |||||||||
fresh | aged | |||||||||
3 | COX5A | Cytochrome c oxidase subunit 5A, mitochondrial | Score: 63 Mass: 16752 pI: 6.30 Coverage: 11 % | Mitochondria | Down Regulated | PR | PR | OR | Ribosome biogenesis; mitochondrial respiratory chain | Breast [77]; cervix [78]; colon [77]; gastric [31]; Kidney [79]; Lung [80–82]; Nasopharyngeal [32]; Oesophageal [77]; Ovarian [77]; Prostate [77]; Thyroid [83] |
19 | COF2 | Cofilin‐2 | Score: 127 Mass: 18725 pI: 7.66 Coverage: 39 % | Cytoplasm, cytoskeleton | Down Regulated | R | R | OR | Actin polymerization | |
34 | 1433G | 14‐3‐3 protein gamma | Score: 315 Mass: 28285 pI: 4.80 Coverage: 44 % | Cytoplasm | Down Regulated | PR | PR | OR | Adapter protein | Breast [86] |
41 | MARE1 | Microtubule‐associated protein RP/EB family member 1 | Score: 62 Mass: 29980 pI: 5.02 Coverage: 29 % | Cytoplasm | Down Regulated | - | R | OR | Microtubule cytoskeleton dynamics; cell migration. | |
50 | NPM | Nucleophosmin | Score: 68 Mass: 32555 pI: 4.64 Coverage: 26 % | Nucleoplasm | Down Regulated | - | R | OR | Chaperone; ribosome biogenesis; p53 and ARF regulation | Bladder [91]; breast [92]; Colon [93]; gastric [94]; haematopoietic [40]; ovarian [95]; prostate [96] |
51 | ANXA1 | Annexin A1 | Score: 369 Mass: 38690 pI: 6.57 Coverage: 53 % | Nucleus, cytoplasm | Down Regulated | R | R | OR | Calcium/phospholipid-binding protein | breast [97]; gastric [98]; HNSC [36]; lung [99]; oesophageal [100, 101]; prostate [100, 102]; |
62 | RSSA | 40S ribosomal protein SA | Score: 311 Mass: 32833 pI: 4.79 Coverage: 34 % | Cell membrane, cytoplasm, nucleus | Up Regulated | R | R | - | laminin receptor; fate determination; tissue morphogenesis | Breast [103]; cervical [104]; colon [54, 55, 105]; melanoma [106] |
65 | ACTB | Actin, cytoplasmic 1 | Score: 767 Mass: 41710 pI: 5.29 Coverage: 53 % | Cytoplasm | Up Regulated | PR | R | PR | cell motility | |
70 | CALU | Calumenin | Score: 141 Mass: 37084 pI: 4.47 Coverage: 14 % | Sarcoplasmic, endoplasmic reticulum | Up Regulated | - | OR | PR | Vitamin K-dependent carboxylation | Colon [111]; gastric [112]; glioblastoma [113]; prostate [114]; |
72 | HNRPF | Heterogeneous nuclear ribonucleoprotein F | Score: 342 Mass: 45643 pI: 5.38 Coverage: 33 % | Nucleus | Down Regulated | R | OR | R | processing of pre-mRNAs; alternative splicing events | |
76 | ENOA | Alpha‐enolase | Score: 471 Mass: 47139 pI: 7.01 Coverage: 47 % | Cytoplasm | Down Regulated | - | PR | R | Glycolysis; hypoxia tolerance; tumour suppressor | |
85 | ATPA | ATP synthase subunit alpha, mitochondrial | Score: 391 Mass: 59714 pI: 9.16 Coverage: 29 % | Mitochondria | Up Regulated | - | OR | OR | Production of ATP from ADP | Breast [120]; colon [121]; gastric [122]; leukemia [123]; thyroid [124]; |
92 | PDIA3 | Protein disulfide-isomerase A3 | Score: 525 Mass: 56747 pI: 5.98 Coverage: 54 % | Endoplasmic reticulum | Down Regulated | PR | PR | R | endopeptidase; electron carrier; | Lung [125]; ovarian [48]; Prostate [46]; retinoblastoma [126] |
100 | HNRPK | Heterogeneous nuclear ribonucleoprotein K | Score: 123 Mass: 50944 pI: 5.39 Coverage: 26 % | Cytoplasm, nucleus | Up Regulated | - | OR | - | Pre-mRNA-binding proteins; p53 response to DNA damage | Breast [127]; colon [128]; Leukemia [129, 130]; lung [131]; Oropharyngeal [132]; pancreatic [133]; |
103 | CH60 | 60 kDa heat shock protein, mitochondrial | Score: 762 Mass: 61016 pI: 5.70 Coverage: 39 % | Mitochondria | Up Regulated | PR | OR | PR | macromolecular assembly; stress working chaperone | |
104 | TCPQ | T‐complex protein 1 subunit theta | Score: 200 Mass: 59583 pI: 5.42 Coverage: 24 % | Cytoplasm | Down Regulated | R | OR | R | chaperone | |
127 | GRP75 | Stress‐70 protein, mitochondrial | Score: 839 Mass: 73635 pI: 5.87 Coverage: 33 % | Mitochondria, nucleus | Down Regulated | R | PR | R | Cell proliferation; cellular aging; chaperone | Brain, Breast, colon, kidney, lung, ovarian [140] |
139 | GLU2B | Glucosidase 2 subunit beta | Score: 80 Mass: 59388 pI: 4.33 Coverage: 11 % | Endoplasmic reticulum | Up Regulated | OR | OR | - | Regulatory subunit of glucosidase II | |
149 | HSP74 | Stress‐70 protein, mitochondrial | Score: 187 Mass: 94271 pI: 5.11 Coverage: 10 % | Cytoplasm | Down Regulated | - | OR | PR | Stress response; cell proliferation, differentiation | Breast [143] |
170 | HNRPC | Heterogeneous nuclear ribonucleoproteins | Score: 142 Mass: 33650 pI: 4.95 Coverage: 8 % | Nucleus | Up Regulated | - | R | OR | mRNA processing and translation | |
215 | CALR | Calreticulin | Score: 246 Mass: 48112 pI: 4.29 Coverage: 17 % | Endoplasmic, sarcoplasmic reticulum | Up Regulated | - | R | OR | Calcium-binding chaperone | Bladder [69]; breast [148]; colon [149]; gastric [150]; glioblastoma [69]; liver [151]; ovarian [69]; pancreas [152]; prostate [153] |
246 | VIME | Vimentin | Score: 539 Mass: 53619 pI: 5.06 Coverage: 81 % | Cytoplasm | Up Regulated | P | OR | - | Filaments attached to nucleus and endoplasmic reticulum |