Fig. 2

TBX3 expression is increased in 21NT cell transfectants. a qRT-PCR with primer sets which recognize both isoforms of TBX3 show total TBX3 mRNA is increased in 21NT cells after stable transfection with either TBX3iso1 or TBX3iso2. b qRT-PCR with TBX3iso1 specific primers show TBX3iso1 mRNA is increased in 21NT cells after stable transfection with TBX3iso1. c qRT-PCR with TBX3iso2 specific primers show TBX3iso2 mRNA is increased in 21NT cells after stable transfection with TBX3iso2. d Western blot showing total TBX3 protein is increased in the 21NT cells after stable transfection with either TBX3iso1 or TBX3iso2. e Densitometry quantification of Western blot shows significantly higher TBX3 protein expression in both the TBX3iso1 and TBX3iso2 transfectants. Means were analyzed using one-way ANOVA followed by Tukey’s post hoc test and p < 0.05 was considered statistically significant; * p < 0.05, ***p < 0.001. Error bars indicate Standard Error. Results of the qRT-PCR and Western blot are representative of at least three independent experiments. f Quantification of percentage of nuclei staining positive by immunohistochemistry for TBX3 in 21NT transfectant cell pellets. g Immunohistochemistry for TBX3 in 21NT transfectant cell pellets. TBX3iso1 and TBX3iso2 vs. vector control show increased amount of protein, which is mainly nuclear-localized