Fig. 4

DOT1L silencing induces MET and CD24 mRNA expression in MDA-MB-231 in vitro. a Quantitative and qualitative analysis after transfection with DOT1L SMARTpool. b Quantitative and qualitative analysis after transfection with four individual siRNA and the DOT1L SMARTpool in MDA-MB-231 cells. Individual siRNAs significantly induced E-CAD (siRNA 1, 2) and morphological changes (siRNA 2) compared with siScr transfected cells. Data are presented as the mean ± sd of 2 independent experiments each with 3 biological replicates (* p < 0.05, **p < 0.01, ***p < 0.001 compared with siScr determined by Wilcoxon-Mann Whitney’s test). c RT-qPCR quantification of DOT1L, E-CAD and CD24 transcripts using specific primers in MDA-MB-231 cells transfected with individual siRNA 1 (10 nM) and 2 (0.1 nM). The columns represent the mean expression ± sd of 2 independent experiments normalized to siScr. d Individual siRNA 1 (10 nM) reduced the migration compared with siScr or miR-200c transfected cells. Data are presented as the mean ± sd of 2 independent experiments each with 2 biological replicates. Scale bars, 100 μm