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Fig. 1 | BMC Cancer

Fig. 1

From: Inhibition of nuclear factor-kappa B enhances the tumor growth of ovarian cancer cell line derived from a low-grade papillary serous carcinoma in p53-independent pathway

Fig. 1

Genetic specific of ovarian cancer cells. a Sequence of KRAS gene in HOC-7 and relative transgenic cells. b The expression of kras, braf, p21 and p53 in HOC-7 and relative transgenic tumor tissues by immunohistochemical staining (400×). c Electrophoretic mobility shift assays showing that the binding of NF-kB to its promoter DNA consensus sequence was reduced in IkBαM-transfected cells. d Luciferase-reporter assays showing the reduced transcriptional activity of NF-kB. Error bars =95 % confidence intervals. Blank represents the empty control vector, wild type represents the luciferase reporter vector, which has an HIV promoter containing binding sites to NF-kB protein. The intensity of luciferase activity thus represents the cellular NF-kB activity. Mutant represents the luciferase reporter vector with NF-kB binding sites eliminated. Ectopic expression of IkBαM markedly reduced the reporter activity, indicative of dampened cellular NF-kB activity

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BMC Cancer

ISSN: 1471-2407

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