Fig. 3

Effects of MK-2206, cisplatin, and olaparib on AKT activity in BRCA wild-type and mutant EOC cells. a PEO1 and PEO4 cells were treated with 0.3 μM, 1 μM, or 3 μM MK-2206 (MK) for 24 h. Band intensities quantified with ImageJ software. b 1. PEO1 and PEO4 cells were treated with 0.625 μM, 1.25 μM, or 2.5 μM cisplatin (Cis) for 24 h. 2. PEO1 and PEO4 cells were treated with 2.5 μM cisplatin (Cis) for 0, 3, 6, 12, and 24 h. c PEO1 and PEO4 cells were treated with 0.625 μM, 1.25 μM, or 2.5 μM olaparib (AZD) for 24 h. Total proteins were assessed for the levels of phosphorylated and total AKT and S6 by western blot analysis. HSC-70 protein levels were also used as a loading control. The ratios of phosphorylated protein to total protein relative to that of the control of PEO1 (set as 1) are shown in bar graphs