Antigen | Specificities | Purchaser | Dilution | Pre-treatment |
---|---|---|---|---|
PER1 (Per12-A) | Polyclonal | AH Diagnostics AS Fjellgata 1, Oslo | 1:50, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
PER2 (N-19, sc-7728) | Polyclonal | Santa Kruz Biotecnology Inc. Europe | 1:200, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
PER3 (Per32-A) | Polyclonal | AH Diagnostics AS Fjellgata 1, Oslo | 1:50, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
CRY1 (W-L5, sc-101006) | Monoclonal | Santa Kruz Biotecnology Inc. Europe | 1:200, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
CRY2 (P-21, sc-130731) | Polyclonal | Santa Kruz Biotecnology Inc. Europe | 1:200, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
BMAL 1 (LS-B660/12275) | Polyclonal | Lifespan Biosciences (Nordic biosite) | 1:100, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
CLOCK (LS-B278/18928 | Polyclonal | Lifespan Biosciences (Nordic biosite) | 1:500, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
Anti-CSNK1α1L | Polyclonal | Abcam.com England | 1:150, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
Casein kinase 1Ɛ (Sc-25423) | Polyclonal | Santa Kruz Biotecnology Inc. Europe | 1:100, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |
Casein kinase 1α (Sc-28886) | Polyclonal | Santa Kruz Biotecnology Inc. Europe | 1:100, overnight at 4 °C | Microwave treatment for 10 min at 750 W and 20 min at 500 W in 10 mmol/L citrate buffer pH6 |