Fig. 2

FACS analysis and immunohistochemistry of xenograft ALDH⁺ HCT-116 cell tumors treated with LTD₄ or PGE₂. a Representative dot plots and corresponding graphs of FACS analysis of the percentage of ALDH⁺ cells in dissociated tumors from vehicle (ethanol), LTD₄ or PGE₂ treated groups respectively. b–e Immunohistochemical analysis of ALDH, Dclk1, β-catenin, and COX-2 protein expression levels. The tumors from vehicle- (ethanol), LTD₄- and PGE₂-treated mice were processed for immunohistochemical analysis. Representative images (40×) and corresponding bar graphs show staining scores of (b) ALDH, (c) Dclk1, (d) β-catenin, and (e) COX-2 proteins in tumors. The final scores represent the sum of the staining intensity and staining percentage within tumor areas. The data are expressed as means ± SEM, n = 6 mice in each group. *P < 0.05, **P < 0.01 and ***P < 0.001