Fig. 3
From: Selective mode of action of plumbagin through BRCA1 deficient breast cancer stem cells
Cytotoxicity of PB is selective to BCSCs from BRCA1-defective breast cancer cell line. Effects of (a) Plumbagin and (b) Carboplatin on the proliferation of HCC1937 and HCC1937/wt BRCA1 cells. c PB reduces the sphere forming efficiency in terms of size and (d) number of spheres formed in a dose dependent manner in HCC1937 cells when compared to HCC1937/wt BRCA1 cells. Scale = 100 μm. e Reduction in the ALDH1+ population after PB treatment observed in HCC1937 cells while an enrichment of ALDH1+ stem cells upon PB treatment was observed in HCC1937/wt BRCA1 cells by flow cytometry and graph showing the variation in the percentages of ALDH1+ populations harbored by the breast cancer cell lines after treatment with ≤ IC50/2 (1 μM ) and ≤ IC50/4 (0.5 μM) concentrations of PB