Fig. 8
From: Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells
Detection of caspases activity in response to miR-21 silencing. SiHa (panel a) and HeLa (panel b) cells were analyzed by caspase-3/7 activities, 48 h after of non-treated (lane NT), treated with H2O2 (apoptosis control), transfected with empty vector (pSilencer 1.0-U6) and transfected with pSIMIR21 plasmids. DMEM medium alone was included. Following transfection, luciferase activity levels were measured and luminescence value corresponded to relative light units. The values are presented as mean ± SD and P values <0.01 are indicated with asterisks. Each assay was carried out three separate times