Fig. 4
From: Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells
Functional analysis of MREs recognition sequences of PTEN gene by miR-21. Panel a. Nucleotide sequences of MRE21-1 and MRE21-2 of PTEN 3′-UTR regulatory region. MicroRNAs response elements of miR-21 (MRE21-1 of 1628 to 1650 nt and MRE21-2 of 1925 to 1965 nt) and complementary with miR-21 are indicated. Information was generated from nucleotide sequence database for PTEN [GeneBank: NM_000314.4] and for hsa-miR-21 [GeneBank: MI0000077]. Panel b. Regulation of PTEN 3′-UTR region modulated by miR-21 microRNA in SiHa cells non transfected (lane 1) or transfected with pSIMIR21 (lane 2), with pMIR-Report-Luciferase empty vector (lane 3), with pMRE21PtenLuc1 (lane 4), with pMRE21PtenLuc1 and pSIMIR21 (lane 5), with pMRE21PtenLuc2 (lane 6) and pMRE21PtenLuc2 and pSIMIR21 (lane 7) plasmids. After 48 h of transfection, luciferase activity levels were measured. Data shown represent the average of four independent experiments